A set of Columbia-0-specific single nucleotide polymorphism markers for the genetic analysis of natural variation in Arabidopsis thaliana
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چکیده
منابع مشابه
Application of single-nucleotide polymorphism (SNP) as a molecular marker in the study of genetic diversity of aquatic populations
Genetic diversity is one of the important and essential characteristics of any population for its survival. The study of genetic variation in different populations of aquatic organisms is of particular importance in order to protect, stabilize and manage their stocks. Based on studies conducted in recent years, molecular markers have proven that they can be used as indicators of the genetic div...
متن کاملMarker development for the genetic study of natural variation in Arabidopsis thaliana
We report AtPRIMER, an application that automates the discovery of new polymorphic markers between ecotypes of Arabidopsis thaliana. On specifying two ecotypes and the genomic region of interest, the script retrieves all corresponding single nucleotide polymorphisms (SNPs) and generates CAPS and/or dCAPS PCR primer sequences. We show that AtPRIMER accurately found specific polymorphic markers f...
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The potential of different DNA based molecular markers was examined for the detection of single nucleotide polymorphism (SNP) in the waxy gene and a microsatellite (SSR) sequence closely linked to it in a collection of rice varieties. DNA was extracted from leaf samples of 68 different rice cultivars by the CTAB method and specific primers were designed for the amplification of waxy gene and SS...
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Background Single nucleotide polymorphism (SNPs) are considered as one of the underlying causes of male infertility. Proper sperm chromatin packaging which involves replacement of histones with protamines has profound effect on male fertility. Over 20 SNPs have been reported for the protamine 1 and 2. MaterialsAndMethods The aim of this study was to evaluate the frequency of two previously repo...
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Genetic markers such as single nucleotide polymorphisms (SNPs) are essential tools for positional cloning, association, or quantitative trait locus mapping and the determination of genetic relationships between individuals. We identified and characterized a genome-wide set of SNP markers by generating 10,706 expressed sequence tags (ESTs) from cDNA libraries derived from 6 different accessions,...
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